Pseudorabies virus (gB) pahu ʻike PCR

ʻO ka wehewehe pōkole:

Hoʻohana kēia kit i ke ʻano PCR fluorescent manawa maoli e ʻike ai i ka RNA o Pseudorabies virus (gB gene) (PRV) i nā mea maʻi maʻi e like me nā tonsils, lymph nodes a me nā mea maʻi maʻi a me ka wai e like me ke kano a me ke koko o nā puaʻa.


Huahana Huahana

Huahana Huahana

inoa mea kūʻai

Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)

Nui

48 ho'āʻo/kit, 50 hoʻāʻo/kit

Hoohana Manao

Hoʻohana kēia kit i ke ʻano PCR fluorescent manawa maoli e ʻike ai i ka RNA o Pseudorabies virus (gB gene) (PRV) i nā mea maʻi maʻi e like me nā tonsils, lymph nodes a me nā mea maʻi maʻi a me ka wai e like me ke kano a me ke koko o nā puaʻa.He kūpono ia no ka ʻike, ʻike a me ka noiʻi epidemiological o Pseudorabies virus (gB gene).ʻO ka kit he ALL-READY PCR SYSTEM (Lyophilized), nona ka DNA amplification enzyme, reaction buffer, nā kumu mua a me nā ʻimi noiʻi e pono ai no ka ʻike ʻana i ka RT-PCR fluorescent.

ʻIke huahana

Nā ʻāpana Pūʻolo kikoʻī Mea hoʻohui
PRV PCR Mix 1 × ʻōmole (Lyophilized pauda)  50 Hoao nā dNTP, MgCl2, Nā mea mua, Probes, Taq DNA polymerase
6×0.2ml 8 paipu paipu(Lyophilized) 48 Hoao
Manaʻo maikaʻi 1*0.2ml paipu (lyophilized)  10 Na hoao

Plasmid a i ʻole Pseudovirus i loaʻa nā ʻāpana kikoʻī PRV(gB).

Hoʻoheheʻe ʻana 1.5 ml Cryotube 500uL /
Manaʻo ʻino 1.5 ml Cryotube 200uL 0.9%NaCl

Waihona & Ola

(1) Hiki ke lawe ʻia ka pahu ma ka lumi wela.

(2) He 18 mahina ka noho ana ma -20 ℃ a me 12 mahina ma 2 ℃ ~ 30 ℃.

(3) E nānā i ka lepili ma ka pahu no ka lā hana a me ka lā pau.

(4) Pono e mālama ʻia ka lyophilized powder version reagent ma -20 ℃ ma hope o ka hoʻoheheʻe ʻana a me ka hoʻokuʻu hou ʻana - pono e emi iho ma mua o 4 mau manawa.

Nā mea kani

GENECHECKER UF-150, UF-300 mea kani PCR fluorescence manawa maoli.

Kiʻi hana

a) ʻO ka ʻōmole:

1

b) 8 mana o ka paipu punawai:

2

Pcr Amplification

Hoʻonohonoho ʻia

ʻanuʻu

Kaapuni

Mahana (℃)

Manawa

Kaila fluorescence

1

1

95

2min

/

2

40

95

5s

/

60

10s

E hōʻiliʻili i ka fluorescence FAM

*E hoʻomaopopo: E hōʻiliʻili ʻia nā hōʻailona o nā kahawai fluorescence FAM ma 60 ℃.

Ka unuhi ana i na hopena hoao

Kanal

Ka wehewehe ʻana i nā hopena

Kanal FAM

Ct≤35

PRV(gB) Pono

Undet

PRV(gB) ʻino

35

Hoʻopiʻi kānalua, hoʻāʻo hou *

*Inā he Ct waiwai ≤40 ka hopena hoʻāʻo hou o ke kahawai FAM a hōʻike i ka pihi hoʻonui ʻano “S” maʻamau, unuhi ʻia ka hopena ma ke ʻano he maikaʻi, inā ʻaʻole ia he ʻino.


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