ʻImi ʻike Pseudorabies virus (gB) PCR

Hōʻike Pōkole:

Hoʻohana kēia kit i ka hana PCR fluorescent manawa maoli e ʻike i ka RNA o ka maʻi Pseudorabies (gB gene) (PRV) i nā mea maʻi maʻi e like me nā tonsil, nā lymph node a me nā maʻi maʻi a me nā wai e like me ka lāʻau lapaʻau a me ke koko o nā puaʻa.


ʻIke kikoʻī

Nā huahana huahana

inoa mea kūʻai

Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)

Nui

48tests / kit, 50tests / kit

Hoʻohana i manaʻo ʻia

Hoʻohana kēia kit i ka hana PCR fluorescent manawa maoli e ʻike i ka RNA o ka maʻi Pseudorabies (gB gene) (PRV) i nā mea maʻi maʻi e like me nā tonsil, nā lymph node a me nā maʻi maʻi a me nā wai e like me ka lāʻau lapaʻau a me ke koko o nā puaʻa. He kūpono ia no ka ʻike ʻana, ka hōʻoia a me ka hoʻokolokolo ʻana o ka epidemiological o ka maʻi Pseudorabies (gB gene). ʻO ka pahu kini kahi ALL-READY PCR SYSTEM (Lyophilized), kahi i loaʻa ka DNA amplification enzyme, buffer reaction, primers kikoʻī a me nā probes i koi ʻia no ka ʻike RT-PCR fluorescent.

Nā huahana huahana

Nā ʻĀpana Pūʻolo hoakaka Mea hoʻohui
Hoʻohui PRR PCR 1 × ʻōmole (Lyophilized pauka)  50 ho'āʻo dNTPs, MgCl2, Nā Puke Kahiko, ʻIke, Taq DNA polymerase
6 × 0.2ml 8 Tube kaha pono(Lyophilized) 48 Hōʻike
Mana Kūpono 1 * 0.2ml paipu (lyophilized)  10Nā hoʻāʻo

Plasmid a i ʻole Pseudovirus i loaʻa nā ʻāpana kikoʻī PRV (gB)

Hoʻoholo hoʻonā 1.5 ml Cryotube 500uL /
Kaohi maikaʻi ʻole 1.5 ml Cryotube 200uL 0.9% NaCl

Wā mālama & ola ola

(1) Hiki ke lawe i ka pahu i ka mahana o ka lumi.

(2) ʻO 18 ka lōʻihi o ke ola ma -20 ℃ a me 12 mau mahina ma 2 ℃ ~ 30.

(3) E ʻike i ka lepili ma ka pika no ka lā hana a me ka lā pau.

(4) Pono e mālama ʻia ka mana o ka pauka lyophilized ma -20 ℃ ma hope o ka hemo ʻana a me ka paʻahau hou ʻana - ma lalo o 4 mau manawa.

Mea kani

GENECHEKER UF-150, UF-300 PCR pono fluorescence manawa pono.

Kiʻi Hana

a) Mana ʻōmole:

1

b) 8 mana paipu maikaʻi loa:

2

Hoʻonui Pcr

Hoʻonohonoho ʻia

ʻAnuʻu

Pōʻaiapuni

Mahana (℃)

Manawa

Kanal fluorescence

1

1

95

2min

/

2

40

95

5s

/

60

10s

E ʻohiʻohi fluorescence FAM

* Nānā: e hōʻiliʻili ʻia nā hōʻailona o nā kahawai fluorescence FAM ma 60 ℃.

Unuhi i nā hopena hōʻike

Kanal

Ka wehewehe ʻana i nā hopena

Kanal FAM

Ct≤35

PRV (gB) Maikaʻi

Undet

PRV (gB) maikaʻi ʻole

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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