ʻImi ʻike Pseudorabies virus (gB) PCR
inoa mea kūʻai
Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)
Nui
48tests / kit, 50tests / kit
Hoʻohana i manaʻo ʻia
Hoʻohana kēia kit i ka hana PCR fluorescent manawa maoli e ʻike i ka RNA o ka maʻi Pseudorabies (gB gene) (PRV) i nā mea maʻi maʻi e like me nā tonsil, nā lymph node a me nā maʻi maʻi a me nā wai e like me ka lāʻau lapaʻau a me ke koko o nā puaʻa. He kūpono ia no ka ʻike ʻana, ka hōʻoia a me ka hoʻokolokolo ʻana o ka epidemiological o ka maʻi Pseudorabies (gB gene). ʻO ka pahu kini kahi ALL-READY PCR SYSTEM (Lyophilized), kahi i loaʻa ka DNA amplification enzyme, buffer reaction, primers kikoʻī a me nā probes i koi ʻia no ka ʻike RT-PCR fluorescent.
Nā huahana huahana
| Nā ʻĀpana | Pūʻolo | hoakaka | Mea hoʻohui |
| Hoʻohui PRR PCR | 1 × ʻōmole (Lyophilized pauka) | 50 ho'āʻo | dNTPs, MgCl2, Nā Puke Kahiko, ʻIke, Taq DNA polymerase |
| 6 × 0.2ml 8 Tube kaha pono(Lyophilized) | 48 Hōʻike | ||
| Mana Kūpono | 1 * 0.2ml paipu (lyophilized) | 10Nā hoʻāʻo |
Plasmid a i ʻole Pseudovirus i loaʻa nā ʻāpana kikoʻī PRV (gB) |
| Hoʻoholo hoʻonā | 1.5 ml Cryotube | 500uL | / |
| Kaohi maikaʻi ʻole | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Wā mālama & ola ola
(1) Hiki ke lawe i ka pahu i ka mahana o ka lumi.
(2) ʻO 18 ka lōʻihi o ke ola ma -20 ℃ a me 12 mau mahina ma 2 ℃ ~ 30.
(3) E ʻike i ka lepili ma ka pika no ka lā hana a me ka lā pau.
(4) Pono e mālama ʻia ka mana o ka pauka lyophilized ma -20 ℃ ma hope o ka hemo ʻana a me ka paʻahau hou ʻana - ma lalo o 4 mau manawa.
Mea kani
GENECHEKER UF-150, UF-300 PCR pono fluorescence manawa pono.
Kiʻi Hana
a) Mana ʻōmole:
b) 8 mana paipu maikaʻi loa:
Hoʻonui Pcr
Hoʻonohonoho ʻia
|
ʻAnuʻu |
Pōʻaiapuni |
Mahana (℃) |
Manawa |
Kanal fluorescence |
|
1 |
1 |
95 |
2min |
/ |
|
2 |
40 |
95 |
5s |
/ |
|
60 |
10s |
E ʻohiʻohi fluorescence FAM |
* Nānā: e hōʻiliʻili ʻia nā hōʻailona o nā kahawai fluorescence FAM ma 60 ℃.
Unuhi i nā hopena hōʻike
|
Kanal |
Ka wehewehe ʻana i nā hopena |
|
Kanal FAM |
|
|
Ct≤35 |
PRV (gB) Maikaʻi |
|
Undet |
PRV (gB) maikaʻi ʻole |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
