E.coli O157: H7 PCR ʻike kit

Hōʻike Pōkole:

ʻO Escherichia coli O157: ʻo H7 (E.coli O157: H7) kahi bacterium maikaʻi ʻole e pili ana i ka genus Enterobacteriaceae, kahi e hua ai ka nui o Vero toxin.


ʻIke kikoʻī

Nā huahana huahana

inoa mea kūʻai

E.coli O157: H7 PCR ʻike kit (Lyophilized)

Nui

48tests / kit, 50tests / kit

Hoʻohana i manaʻo ʻia

ʻO Escherichia coli O157: ʻo H7 (E.coli O157: H7) kahi bacterium maikaʻi ʻole e pili ana i ka genus Enterobacteriaceae, kahi e hua ai ka nui o Vero toxin. Clinically, hana mau ia me ka ʻeha o ka ʻōpū nui a me ka wai o ka diarrhea, a ukali ʻia e ka maʻi hemorrhagic i kekahi mau lā ma hope aku, ka mea e hiki ai i ka fiva a i ʻole ke kuni, a me ka make i nā hihia koʻikoʻi. Kūpono kēia kini no ka ʻike kūpono o Escherichia coli O157: H7 i ka meaʻai, nā wai, nā feces, ka luaʻi, ka wai hoʻoliʻiliʻi bacteria a me nā mea ʻē aʻe e hoʻohana ana i ke kumumanaʻo o PCR manawa maoli. Lyophilized), i loaʻa ka DNA hoʻonui i ka enzyme, buffer reaction, primers kikoʻī a me nā probes e pono ai no ka ʻike ʻana o fluorescent PCR ..

Nā huahana huahana

Nā ʻĀpana Pūʻolo hoakaka Mea hoʻohui
E.coli O157: H7 PCR Hoʻohui 1 × ʻōmole (Lyophilized pauka)  50 ho'āʻo dNTPs, MgCl2, Nā Puke Kahiko, ʻIke, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 Tube kaha pono(Lyophilized) 48 Hōʻike
Mana Kūpono 1 * 0.2ml paipu (lyophilized)  10Nā hoʻāʻo

Plasmid i loaʻa iā E.coli O157: H7 ʻāpana ʻāpana

Hoʻoholo hoʻonā 1.5 ml Cryotube 500uL /
Kaohi maikaʻi ʻole 1.5 ml Cryotube 200uL 0.9% NaCl

Wā mālama & ola ola

(1) Hiki ke lawe i ka pahu i ka mahana o ka lumi.

(2) ʻO 18 ka lōʻihi o ke ola ma -20 ℃ a me 12 mau mahina ma 2 ℃ ~ 30.

(3) E ʻike i ka lepili ma ka pika no ka lā hana a me ka lā pau.

(4) Pono e mālama ʻia ka mana o ka pauka lyophilized ma -20 ℃ ma hope o ka hemo ʻana a me ka paʻahau hou ʻana - ma lalo o 4 mau manawa.

Mea kani

GENECHEKER UF-150, UF-300 PCR pono fluorescence manawa pono.

Kiʻi Hana

a) Mana ʻōmole:

1

b) 8 mana paipu maikaʻi loa:

2

Hoʻonui Pcr

Paipai ʻia Ke kau nei

ʻAnuʻu     Pōʻaiapuni Mahana (℃)   Manawa Kanal fluorescence
1 1 95 2min  
2 40 95 5s  
60 10s E ʻohiʻohi fluorescence FAM

* Nānā: e hōʻiliʻili ʻia ka hōʻailona o ke kahawai fluorescence FAM ma 60 ℃.

Unuhi i nā hopena hōʻike

Kanal

Ka wehewehe ʻana i nā hopena

Kanal FAM

Ct≤35

E.coli O157: H7 Maikaʻi

Undet

E.coli O157: H7 maikaʻi ʻole

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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