E.coli O157: H7 PCR ʻike kit
inoa mea kūʻai
E.coli O157: H7 PCR ʻike kit (Lyophilized)
Nui
48tests / kit, 50tests / kit
Hoʻohana i manaʻo ʻia
ʻO Escherichia coli O157: ʻo H7 (E.coli O157: H7) kahi bacterium maikaʻi ʻole e pili ana i ka genus Enterobacteriaceae, kahi e hua ai ka nui o Vero toxin. Clinically, hana mau ia me ka ʻeha o ka ʻōpū nui a me ka wai o ka diarrhea, a ukali ʻia e ka maʻi hemorrhagic i kekahi mau lā ma hope aku, ka mea e hiki ai i ka fiva a i ʻole ke kuni, a me ka make i nā hihia koʻikoʻi. Kūpono kēia kini no ka ʻike kūpono o Escherichia coli O157: H7 i ka meaʻai, nā wai, nā feces, ka luaʻi, ka wai hoʻoliʻiliʻi bacteria a me nā mea ʻē aʻe e hoʻohana ana i ke kumumanaʻo o PCR manawa maoli. Lyophilized), i loaʻa ka DNA hoʻonui i ka enzyme, buffer reaction, primers kikoʻī a me nā probes e pono ai no ka ʻike ʻana o fluorescent PCR ..
Nā huahana huahana
| Nā ʻĀpana | Pūʻolo | hoakaka | Mea hoʻohui |
| E.coli O157: H7 PCR Hoʻohui | 1 × ʻōmole (Lyophilized pauka) | 50 ho'āʻo | dNTPs, MgCl2, Nā Puke Kahiko, ʻIke, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 Tube kaha pono(Lyophilized) | 48 Hōʻike | ||
| Mana Kūpono | 1 * 0.2ml paipu (lyophilized) | 10Nā hoʻāʻo |
Plasmid i loaʻa iā E.coli O157: H7 ʻāpana ʻāpana |
| Hoʻoholo hoʻonā | 1.5 ml Cryotube | 500uL | / |
| Kaohi maikaʻi ʻole | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Wā mālama & ola ola
(1) Hiki ke lawe i ka pahu i ka mahana o ka lumi.
(2) ʻO 18 ka lōʻihi o ke ola ma -20 ℃ a me 12 mau mahina ma 2 ℃ ~ 30.
(3) E ʻike i ka lepili ma ka pika no ka lā hana a me ka lā pau.
(4) Pono e mālama ʻia ka mana o ka pauka lyophilized ma -20 ℃ ma hope o ka hemo ʻana a me ka paʻahau hou ʻana - ma lalo o 4 mau manawa.
Mea kani
GENECHEKER UF-150, UF-300 PCR pono fluorescence manawa pono.
Kiʻi Hana
a) Mana ʻōmole:
b) 8 mana paipu maikaʻi loa:
Hoʻonui Pcr
Paipai ʻia Ke kau nei
| ʻAnuʻu | Pōʻaiapuni | Mahana (℃) | Manawa | Kanal fluorescence |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | E ʻohiʻohi fluorescence FAM |
* Nānā: e hōʻiliʻili ʻia ka hōʻailona o ke kahawai fluorescence FAM ma 60 ℃.
Unuhi i nā hopena hōʻike
|
Kanal |
Ka wehewehe ʻana i nā hopena |
|
Kanal FAM |
|
|
Ct≤35 |
E.coli O157: H7 Maikaʻi |
|
Undet |
E.coli O157: H7 maikaʻi ʻole |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
