中文E.coli O157:H7 PCR ʻike kime
inoa mea kūʻai
E.coli O157:H7 PCR pahu ʻike (Lyophilized)
Nui
48 ho'āʻo/kit, 50 hoʻāʻo/kit
Hoohana Manao
ʻO Escherichia coli O157:H7 (E.coli O157:H7) he huakino gram-negative no ka genus Enterobacteriaceae, nāna e hoʻopuka i ka nui o Vero toxin.ʻO ka maʻi maʻi, hiki koke mai me ka ʻeha ʻōpū nui a me ka maʻi maʻi wai, a ukali ʻia e ka hemorrhagic diarrhea i kekahi mau lā ma hope, hiki ke alakaʻi i ke kuni a i ʻole ke kuni, a me ka make i nā hihia koʻikoʻi.He kūpono kēia kit no ka ʻike qualitative o Escherichia coli O157:H7 i ka meaʻai, nā wai wai, nā feces, luaʻi, ka wai hoʻonui i ka bacteria a me nā laʻana ʻē aʻe e hoʻohana ana i ke kumu o ka PCR manawa maoli. Lyophilized), ka mea i loaʻa i ka DNA amplification enzyme, reaction buffer, nā kumu mua a me nā ʻimi noiʻi e pono ai no ka ʻike PCR fluorescent.
ʻIke huahana
| Nā ʻāpana | Pūʻolo | kikoʻī | Mea hoʻohui |
| E.coli O157:H7 PCR Mix | 1 × ʻōmole (Lyophilized pauda) | 50 Hoao | nā dNTP, MgCl2, Nā mea mua, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6×0.2ml 8 paipu paipu(Lyophilized) | 48 Hoao | ||
| Manaʻo maikaʻi | 1*0.2ml paipu (lyophilized) | 10 Na hoao | Plasmid i loaʻa iā E.coli O157:H7 mau ʻāpana kikoʻī |
| Hoʻoheheʻe ʻana | 1.5 ml Cryotube | 500uL | / |
| Manaʻo ʻino | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Waihona & Ola
(1) Hiki ke lawe ʻia ka pahu ma ka lumi wela.
(2) He 18 mahina ka noho ana ma -20 ℃ a me 12 mahina ma 2 ℃ ~ 30 ℃.
(3) E nānā i ka lepili ma ka pahu no ka lā hana a me ka lā pau.
(4) Pono e mālama ʻia ka lyophilized powder version reagent ma -20 ℃ ma hope o ka hoʻoheheʻe ʻana a me ka hoʻokuʻu hou ʻana - pono e emi iho ma mua o 4 mau manawa.
Nā mea kani
GENECHECKER UF-150, UF-300 mea kani PCR fluorescence manawa maoli.
Kiʻi hana
a) ʻO ka ʻōmole:
b) 8 mana o ka paipu punawai:
Pcr Amplification
Paipai ʻiaHoʻonohonoho
| ʻanuʻu | Kaapuni | Mahana (℃) | Manawa | Kaila fluorescence |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | E hōʻiliʻili i ka fluorescence FAM |
* Nānā: E hōʻiliʻili ʻia ka hōʻailona o ke kahawai fluorescence FAM ma 60 ℃.
Ka unuhi ana i na hopena hoao
| Kanal | Ka wehewehe ʻana i nā hopena |
| Kanal FAM | |
| Ct≤35 | E.coli O157:H7 Pono |
| Undet | E.coli O157:H7 ʻino |
| 35 | Hoʻopiʻi kānalua, hoʻāʻo hou * |
*Inā he Ct waiwai ≤40 ka hopena hoʻāʻo hou o ke kahawai FAM a hōʻike i ka pihi hoʻonui ʻano “S” maʻamau, unuhi ʻia ka hopena ma ke ʻano he maikaʻi, inā ʻaʻole ia he ʻino.
