ʻO Listeria monocytogenes PCR Detection Kit
inoa mea kūʻai
ʻO Listeria monocytogenes PCR Detection Kit
Nui
48 ho'āʻo/kit, 50 hoʻāʻo/kit
Hoohana Manao
ʻO Listeria monocytogenes kahi microbacterium gram-positive hiki ke ulu ma waena o 4 ℃ a me 45 ℃.ʻO ia kekahi o nā pathogens nui e hoʻoweliweli i ke olakino o ke kanaka i ka meaʻai i hoʻomoʻa ʻia.ʻO nā hōʻike nui o ka maʻi he septicemia, maningitis a me ka mononucleosis.He kūpono kēia kit no ka ʻike qualitative o Listeria monocytogenes i ka meaʻai, nā ʻano wai, nā feces, ka luaʻi, ka wai hoʻonui i ka bacteria a me nā laʻana ʻē aʻe e hoʻohana ana i ke kumu o ka PCR fluorescence maoli. , i loko o ka DNA amplification enzyme, reverse transcriptase, reaction buffer, nā kumu mua a me nā ʻimi noiʻi e pono ai no ka ʻike ʻana i ka PCR fluorescent.
Waihona & Ola
(1) Hiki ke lawe ʻia ka pahu ma ka lumi wela.
(2) He 18 mahina ka noho ana ma -20 ℃ a me 12 mahina ma 2 ℃ ~ 30 ℃.
(3) E nānā i ka lepili ma ka pahu no ka lā hana a me ka lā pau.
(4) Pono e mālama ʻia ka lyophilized powder version reagent ma -20 ℃ ma hope o ka hoʻoheheʻe ʻana a me ka hoʻokuʻu hou ʻana - pono e emi iho ma mua o 4 mau manawa.
ʻIke huahana
Nā ʻāpana | Pūʻolo | kikoʻī | Mea hoʻohui |
Listeria monocytogenes PCR Mix | 1 × ʻōmole (Lyophilized pauda) | 50 Hoao | nā dNTP, MgCl2, Nā mea mua, Probes, Reverse Transcriptase, Taq DNA polymerase |
6×0.2ml 8 paipu paipu(Lyophilized) | 48 Hoao | ||
Manaʻo maikaʻi | 1*0.2ml paipu (lyophilized) | 10 Na hoao | ʻO Plasmid i loaʻa i nā ʻāpana kikoʻī Listeria monocytogenes |
Hoʻoheheʻe ʻana | 1.5 ml Cryotube | 500uL | / |
Manaʻo ʻino | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Nā mea kani
GENECHECKER UF-150, UF-300 mea kani PCR fluorescence manawa maoli.
Kiʻi hana
a)
b)
Pcr Amplification
Paipai ʻiaHoʻonohonoho
ʻanuʻu | Kaapuni | Mahana (℃) | Manawa | Kaila fluorescence |
1 | 1 | 50 | 8 min | |
2 | 1 | 95 | 2min | |
3 | 40 | 95 | 5s | |
60 | 10s | E hōʻiliʻili i ka fluorescence FAM |
*E hoʻomaopopo: E hōʻiliʻili ʻia nā hōʻailona o nā kahawai fluorescence FAM ma 60 ℃.
Ka unuhi ana i na hopena hoao
Kanal | Ka wehewehe ʻana i nā hopena |
Kanal FAM | |
Ct≤35 | Listeria monocytogenes Pono |
Undet | Listeria monocytogenes ʻino |
35 | Hoʻopiʻi kānalua, hoʻāʻo hou * |
*Inā he Ct waiwai ≤40 ka hopena hoʻāʻo hou o ke kahawai FAM a hōʻike i ka pihi hoʻonui ʻano “S” maʻamau, unuhi ʻia ka hopena ma ke ʻano he maikaʻi, inā ʻaʻole ia he ʻino.