ʻO Listeria monocytogenes PCR Detection Kit

ʻO ka wehewehe pōkole:

ʻO Listeria monocytogenes kahi microbacterium gram-positive hiki ke ulu ma waena o 4 ℃ a me 45 ℃.ʻO ia kekahi o nā pathogens nui e hoʻoweliweli i ke olakino o ke kanaka i ka meaʻai i hoʻomoʻa ʻia.


Huahana Huahana

Huahana Huahana

inoa mea kūʻai

ʻO Listeria monocytogenes PCR Detection Kit

Nui

48 ho'āʻo/kit, 50 hoʻāʻo/kit

Hoohana Manao

ʻO Listeria monocytogenes kahi microbacterium gram-positive hiki ke ulu ma waena o 4 ℃ a me 45 ℃.ʻO ia kekahi o nā pathogens nui e hoʻoweliweli i ke olakino o ke kanaka i ka meaʻai i hoʻomoʻa ʻia.ʻO nā hōʻike nui o ka maʻi he septicemia, maningitis a me ka mononucleosis.He kūpono kēia kit no ka ʻike qualitative o Listeria monocytogenes i ka meaʻai, nā ʻano wai, nā feces, ka luaʻi, ka wai hoʻonui i ka bacteria a me nā laʻana ʻē aʻe e hoʻohana ana i ke kumu o ka PCR fluorescence maoli. , i loko o ka DNA amplification enzyme, reverse transcriptase, reaction buffer, nā kumu mua a me nā ʻimi noiʻi e pono ai no ka ʻike ʻana i ka PCR fluorescent.

Waihona & Ola

(1) Hiki ke lawe ʻia ka pahu ma ka lumi wela.

(2) He 18 mahina ka noho ana ma -20 ℃ a me 12 mahina ma 2 ℃ ~ 30 ℃.

(3) E nānā i ka lepili ma ka pahu no ka lā hana a me ka lā pau.

(4) Pono e mālama ʻia ka lyophilized powder version reagent ma -20 ℃ ma hope o ka hoʻoheheʻe ʻana a me ka hoʻokuʻu hou ʻana - pono e emi iho ma mua o 4 mau manawa.

ʻIke huahana

Nā ʻāpana Pūʻolo kikoʻī Mea hoʻohui
Listeria monocytogenes PCR Mix 1 × ʻōmole (Lyophilized pauda)  50 Hoao nā dNTP, MgCl2, Nā mea mua, Probes, Reverse Transcriptase, Taq DNA polymerase
6×0.2ml 8 paipu paipu(Lyophilized) 48 Hoao
Manaʻo maikaʻi 1*0.2ml paipu (lyophilized)  10 Na hoao

ʻO Plasmid i loaʻa i nā ʻāpana kikoʻī Listeria monocytogenes

Hoʻoheheʻe ʻana 1.5 ml Cryotube 500uL /
Manaʻo ʻino 1.5 ml Cryotube 200uL 0.9%NaCl

Nā mea kani

GENECHECKER UF-150, UF-300 mea kani PCR fluorescence manawa maoli.

Kiʻi hana

a)

1

b)

2

Pcr Amplification

Paipai ʻiaHoʻonohonoho

ʻanuʻu Kaapuni Mahana (℃) Manawa Kaila fluorescence
1 1 50 8 min  
2 1 95 2min  
3 40 95 5s  
60 10s E hōʻiliʻili i ka fluorescence FAM

*E hoʻomaopopo: E hōʻiliʻili ʻia nā hōʻailona o nā kahawai fluorescence FAM ma 60 ℃.

Ka unuhi ana i na hopena hoao

Kanal

Ka wehewehe ʻana i nā hopena

Kanal FAM

Ct≤35

Listeria monocytogenes Pono

Undet

Listeria monocytogenes ʻino

35

Hoʻopiʻi kānalua, hoʻāʻo hou *

*Inā he Ct waiwai ≤40 ka hopena hoʻāʻo hou o ke kahawai FAM a hōʻike i ka pihi hoʻonui ʻano “S” maʻamau, unuhi ʻia ka hopena ma ke ʻano he maikaʻi, inā ʻaʻole ia he ʻino.


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