ʻO Salmonella PCR ʻike kiʻi

ʻO ka wehewehe pōkole:

Aia ʻo Salmonella i ka Enterobacteriaceae a me Gram-negative enterobacteria.ʻO Salmonella kahi maʻi maʻamau i lawe ʻia i ka meaʻai a ke kūlana mua i ka maʻi maʻi maʻi.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

inoa mea kūʻai

ʻO Salmonella PCR ʻIke Kiko (lyophilized)

Nui

48 ho'āʻo/kit, 50 hoʻāʻo/kit

Hoohana Manao

Aia ʻo Salmonella i ka Enterobacteriaceae a me Gram-negative enterobacteria.ʻO Salmonella kahi maʻi maʻamau i lawe ʻia i ka meaʻai a ke kūlana mua i ka maʻi maʻi maʻi.ʻO nā hōʻailona nui o ka make ʻana o ka meaʻai ma muli o Salmonella, ʻo ia ka nausea, ka luaʻi ʻana, ka ʻeha ʻōpū, ke poʻo ʻeha, ke anuanu a me ka ʻōpū.Hoʻohana kēia pahu i ke kumu o ka PCR fluorescent manawa maoli a kūpono no ka ʻike qualitative o Salmonella i ka meaʻai, nā laʻana wai, nā feces, ka luaʻi, a me ka wai hoʻonui.

ʻIke huahana

Nā ʻāpana	Pūʻolo	kikoʻī	Mea hoʻohui
PCR Mix	1 × ʻōmole (Lyophilized pauda)	50 Hoao	nā dNTP, MgCl₂, Nā mea mua, Probes, Taq DNA polymerase
PCR Mix	6×0.2ml 8 paipu paipu(Lyophilized)	48 Hoao	nā dNTP, MgCl₂, Nā mea mua, Probes, Taq DNA polymerase
Manaʻo maikaʻi	1*0.2ml paipu (lyophilized)	10 Na hoao	ʻO Plasmid a i ʻole Pseudovirus i loaʻa nā ʻāpana kikoʻī
Hoʻoheheʻe ʻana	1.5 ml Cryotube	500uL	/
Manaʻo ʻino	1.5 ml Cryotube	100uL	0.9%NaCl

Waihona & Ola

(1) Hiki ke lawe ʻia ka pahu ma ka lumi wela.

(2) He 18 mahina ka noho ana ma -20 ℃ a me 12 mahina ma 2 ℃ ~ 30 ℃.

(3) E nānā i ka lepili ma ka pahu no ka lā hana a me ka lā pau.

(4) Pono e mālama ʻia ka lyophilized powder version reagent ma -20 ℃ ma hope o ka hoʻoheheʻe ʻana a me ka hoʻokuʻu hou ʻana - pono e emi iho ma mua o 4 mau manawa.

Nā mea kani

GENECHECKER UF-150, UF-300 mea kani PCR fluorescence manawa maoli.

Kiʻi hana

a) ʻO ka ʻōmole:

b) 8 mana o ka paipu punawai:

Pcr Amplification

Hoʻonohonoho ʻia

ʻanuʻu	Kaapuni	Mahana (℃)	Manawa	Kaila fluorescence
1	1	95	2min	/
2	40	95	5s	/
2	40	60	10s	E hōʻiliʻili i ka fluorescence FAM

Ka unuhi ana i na hopena hoao

Kanal	Ka wehewehe ʻana i nā hopena
Kanal FAM	Ka wehewehe ʻana i nā hopena
Ct≤35	Salmonella Pono
Undet	ʻO Salmonella Negative
35	Hoʻopiʻi kānalua, hoʻāʻo hou *

*Inā he Ct waiwai ≤40 ka hopena hoʻāʻo hou o ke kahawai FAM a hōʻike i ka pihi hoʻonui ʻano “S” maʻamau, unuhi ʻia ka hopena ma ke ʻano he maikaʻi, inā ʻaʻole ia he ʻino.

Mua: Norovirus (GⅡ) RT-PCR ʻIke Kiko

Aʻe: ʻO Shigella PCR ʻike kime