ʻO Salmonella PCR ʻike kiʻi

ʻO ka wehewehe pōkole:

Aia ʻo Salmonella i ka Enterobacteriaceae a me Gram-negative enterobacteria.ʻO Salmonella kahi maʻi maʻamau i lawe ʻia i ka meaʻai a ke kūlana mua i ka maʻi maʻi maʻi.


Huahana Huahana

Huahana Huahana

inoa mea kūʻai

ʻO Salmonella PCR ʻIke Kiko (lyophilized)

Nui

48 ho'āʻo/kit, 50 hoʻāʻo/kit

Hoohana Manao

Aia ʻo Salmonella i ka Enterobacteriaceae a me Gram-negative enterobacteria.ʻO Salmonella kahi maʻi maʻamau i lawe ʻia i ka meaʻai a ke kūlana mua i ka maʻi maʻi maʻi.ʻO nā hōʻailona nui o ka make ʻana o ka meaʻai ma muli o Salmonella, ʻo ia ka nausea, ka luaʻi ʻana, ka ʻeha ʻōpū, ke poʻo ʻeha, ke anuanu a me ka ʻōpū.Hoʻohana kēia pahu i ke kumu o ka PCR fluorescent manawa maoli a kūpono no ka ʻike qualitative o Salmonella i ka meaʻai, nā laʻana wai, nā feces, ka luaʻi, a me ka wai hoʻonui.

ʻIke huahana

Nā ʻāpana Pūʻolo kikoʻī Mea hoʻohui
PCR Mix 1 × ʻōmole (Lyophilized pauda)  50 Hoao nā dNTP, MgCl2, Nā mea mua, Probes, Taq DNA polymerase
6×0.2ml 8 paipu paipu(Lyophilized) 48 Hoao
Manaʻo maikaʻi 1*0.2ml paipu (lyophilized)  10 Na hoao

ʻO Plasmid a i ʻole Pseudovirus i loaʻa nā ʻāpana kikoʻī

Hoʻoheheʻe ʻana 1.5 ml Cryotube 500uL /
Manaʻo ʻino 1.5 ml Cryotube 100uL 0.9%NaCl

Waihona & Ola

(1) Hiki ke lawe ʻia ka pahu ma ka lumi wela.

(2) He 18 mahina ka noho ana ma -20 ℃ a me 12 mahina ma 2 ℃ ~ 30 ℃.

(3) E nānā i ka lepili ma ka pahu no ka lā hana a me ka lā pau.

(4) Pono e mālama ʻia ka lyophilized powder version reagent ma -20 ℃ ma hope o ka hoʻoheheʻe ʻana a me ka hoʻokuʻu hou ʻana - pono e emi iho ma mua o 4 mau manawa.

Nā mea kani

GENECHECKER UF-150, UF-300 mea kani PCR fluorescence manawa maoli.

Kiʻi hana

a) ʻO ka ʻōmole:

1

b) 8 mana o ka paipu punawai:

2

Pcr Amplification

Hoʻonohonoho ʻia

ʻanuʻu

Kaapuni

Mahana (℃)

Manawa

Kaila fluorescence

1

1

95

2min

/

2

40

95

5s

/

60

10s

E hōʻiliʻili i ka fluorescence FAM

Ka unuhi ana i na hopena hoao

Kanal

Ka wehewehe ʻana i nā hopena

Kanal FAM

Ct≤35

Salmonella Pono

Undet

ʻO Salmonella Negative

35

Hoʻopiʻi kānalua, hoʻāʻo hou *

*Inā he Ct waiwai ≤40 ka hopena hoʻāʻo hou o ke kahawai FAM a hōʻike i ka pihi hoʻonui ʻano “S” maʻamau, unuhi ʻia ka hopena ma ke ʻano he maikaʻi, inā ʻaʻole ia he ʻino.


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